| Parties |
| Uganda(1), (2), (3) | 20-February-2020 |
| Norway | 6-July-2020 |
| Australia, Canada, Colombia, Georgia, Mexico, New Zealand, Saint Lucia | 19-February-2021 |
| Australia, Canada, Costa Rica, Georgia, Japan, Mexico, and New Zealand | 23-February-2021 |
| Australia, Canada, Mexico Republic of Korea, Trinidad and Tobago | 28-May -2021 |
| Canada | 6-June-2021 |
| United Kingdom of Great Britain and Northern Ireland | 18-August-2021 |
| Observers |
| World Health Organization | 31-January-2020 |
| Te Atiawa Manawhenua Ki Te Tau Ihu Trust(1) (2) | 6-February-2020 |
| Business for Nature (1)(2) | 10-February-2020 |
| International Chamber of Commerce | 10-February-2020 |
| OP2B | 10-February-2020 |
| One Earth | 10-February-2020 |
| KBA Partnership | 11 -February-2020 |
| UN-Water (1) (2) and letter of support | 14 -February-2020 |
| BirdLife | 14 -February-2020 |
| WWF (1), (2), (3), (4) | 18 -February-2020 |
| Global Forest Coalition | 19 -February-202 |
| Global Wildlife Conservation | 20-February-2020 |
| Born Free | 20-February-2020 |
| Society for Conservation Biology | 21-February-2020 |
| Friends of the Earth Europe (1), (2), (3) | 21-February-2020 |
| UN Environment Management Group | 21-February-2020 |
| Greenovation Hub and Qingdao Marine Conservation Society | 22-February-2020 |
| BirdLife(1), (3) | 23-February-2020 |
| Convention on the Conservation of Migratory Species of Wild Animals | 24-February-2020 |
| IUCN (1), (2) | 24-February-2020 |
| The Nature Conservancy | 25-February-2020 |
| Outreach Network for Gene Drive Research | 25-February-2020 |
| Civil Society Alliance for Biodiversity Conservation | 25-February-2020 |
| UNEP | 26-February-2020 |
| WCS Wildlife Conservation Society, United Nations Development Programme, World Resources Institute, and Rainforest Foundation Norway (1), (2) | 17-March-2020 |
| Rights of Mother Earth (1), (2) | 28-April-2020 |
| International Coral Reef Initiative (1), (2) | 28-May-2020 |
| Population Matters | 22-June-2020 |
| Rights of Mother Earth | 2-July-2020 |
| Stand Up For Your Rights. | 20-July-2020 |
| Coalition for Conservation Genetics | 22-December-2020 |
| COMTEMA/OLACEFS | 4-February-2021 |
| Deakin University | 11-March-2021 |
| Global Initiative to End Wildlife Crime | 26-March-2021 |
| Brazilian Network of Plant-Pollinator Interactions | 17-April-2021 |
| Maritime Aboriginal Peoples Council | 28-June-2021 |
| EPI Foundation | 1-July-2021 |
| WWF | 19-July-2021 |
| Wildlife Conservation Society | 27 July-2021 |
| The Coalition for Conservation Genetics | 6-August-2021 |
| Conservation International (1), (2) | 6-August-2021 |
| Convention on the Conservation of Migratory Species of Wild Animals (CMS) | 13-August-2021 |
| International Fertilizer Association | 16-August-2021 |
| WWF (1), (2), (3), (4) | 16-August-2021 |
| Born Free | 18-August-2021 |
| BirdLife International Partnership (1),( 2 ), (3) | 18-August-2021 |
| World Federation for Animals | 20-August-2021 |
| Wild Heritage, Griffith University and the Australian Rainforest Conservation Society | 23-August-2021 |
| Earth Law Center, Rights of Nature Sweden, Earth Advocacy Youth, and Rights of Mother Earth | 14-September-2021 |
